Kick-start funding to support independent research projects of young PIs in preparation of an own full research proposal.
We asked for money to sequence 100 RNA-seq triplicate sets. Of those, 35 Physcomitrium triplicates are already planned to be sequenced using LMD/scRNA-seq, to fill remaining gaps in the existing expression profiling data and to develop scRNA-seq as a method for MAdLand (see below).
The remaining 65 triplicate sets are not yet assigned. We anticipate that only a minority of those will be used for Physcomitrium and Marchantia, because of the existing/to be generated data of ca. 150 and ca. 200 sample sets, respectively (see below). Rather, we expect these samples to be primarily used for Anthoceros, the two ferns and the two streptophyte algae.
All 100 triplicate sets, together with existing samples (mainly Physcomitrium) and samples generated by associated grants (Bräutigam/Zachgo for Marchantia; Rensing/Schubert/von Schwartzenberg for the streptophyte algae) will be incorporated into the PEATmoss-based central, web-based expression atlas platform that will be made available to MAdLand members upon login. These, upon publication, will become available to the scientific community at large.
In order to grant the sequencing of desired stages, MAdLand members are entitled to hand in a one-page proposal for organisms and stages they would like to have sequenced from this central pool. In case of only a few samples that are asked for, and if no conflict of interest exists, the proposal will be decided upon by the steering committee within a few weeks. In case of a larger number of samples asked for (more than four), and/or a conflict of interest by members of the steering committee, we will also involve the scientific advisory board in the decision. We anticipate a maximum of four weeks for each of the decisions; proposals can be handed in at any time. Selection criteria to grant funding from the central pool are predominantly general usefulness for MAdLand, for example to expand the expression atlas with needed stages, and feasibility to generate the samples.
All requests for central sequencing that have been granted have to stick to the rules for MAdLand sample preparation, naming and quality control. Samples will be received in Marburg, checked for quality and sent to the sequencing facility.
The submission deadline of the second startup fund was July 1st, 2021. Proposals were reviewed by the Steering Committee and the Scientific Advisory Board. The following proposals were designated:
The submission deadline of the first startup fund was September 1st, 2020. Proposals were reviewed by the Steering Committee and the Scientific Advisory Board. The following proposals were designated: